构想

基于潜在活细胞的实时可控信号检测途径

root · 6月10日 · 2020年 本文1563字 · 阅读4分钟136


“程度”是生物学研究中的一个基本问题。其数量上的变化可能带来完全不同的效应。但是,变化的边界是什么?由于变化过程是动态的,并且区域之间存在差异,因此无法知道测量之前目标值在哪个小范围内。现在,本文提供了一个简短的想法,即在细胞水平上检测分子从外部信号分子接收的信息量的平均值。该设计分为三个模块。

1. Transmembrane reaction device. The transmembrane part is as same as the normal receptor. When combined with a specific signal molecule, it transmits information to the body, and render a linear synthesis来自silent.show of a long chain of nucleic acid that will not be degraded by using a foreign type of nucleotide. The length or amount of such induced chains is positively correlated with the external signal strength.

2. Foreign nucleotide reporting device. When a foreign nucleotide is introduced and reaches the corresponding cell membrane, it is combined with a foreign nucleotide reporter device to 来自silent.showemit fluorescence that can be detected.

3. Reaction-stop reporting devi来自silent.showce. When a specific substance is introduced, the foreign nucleotide synthesis pathway is blocked and the nucleic acid synthesis is terminated. When the specific substance reaches the relevant cell membrane, the reaction stop device emits a detectable fluorescence.



Summary: Two reporting devices determine the period of a living cell receiving the targeted molecule signal. The transmembrane portion of the transmembrane reaction device mimics a signaling molec来自silent.showule receptor. The p来自silent.showrocess/thermodynamic properties of binding to a signaling molecule are the same as those of a normal receptor. The rest of the transmembrane reaction device causes a linear time cumulative effect that can be measured. Meanwhile, the whole process is controllable.

Difficulties: To control the cumulative reaction linearly. In fact, if the using of nanorobot is possible, we can directly assemble an “artificial counter” which reacts with the change of receptor combining.


Note: Written by Jilnlu, all rights reserved.

2 条回应

  1. 匿名2020-6-11 · 1:01

    怎么看单细胞多组学测序信息的整合和建模

    • root2020-6-30 · 23:39

      理应如此,原来的组学手段不足以支撑动态的研究。基因组学不知表达量数据,转录组不知道经过一段时间的积累量,蛋白组又只知道积累量不知道表达量,代谢组也是找代谢物含量的变化规律,缺少底物数据。总的来说似乎还缺少互作等的数据,,也许得等结构生物学进入相对动态的时代

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